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  1. Homologous recombination : methods and protocols

    New York, NY : Humana Press, [2021]

    We hope this book will facilitate the use of both classical and more recent approaches to answer specific questions on HR mechanisms as well as to decipher the function of novel factors involved in HR. We expect that this compilation of protocols elaborated by leading experts in the field will be useful not only to the scientific community working in genome integrity but also to scientists working in other areas such as cancer biology or cell cycle with renovated interests in HR and DSB repair.This volume explores homologous recombination's (HR) essential role in meiotic and somatic cells. It discusses the analysis of different steps of the HR process from the genetic, molecular biology, and cell biology perspectives. Using a variety of model systems, chapters in this book cover topics such as the genome-wide mapping of DNA double-strand breaks (DSB); analysis of DNA-end resection and recombination intermediates by gel electrophoresis and southern blotting; cell-based monitoring of HR activity in meiotic and mitotic cells; in vitro analysis of branch migration, DNA strand exchange and Holliday junction resolution; or super resolution imaging of HR repair at collapsed replication forks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Homologous Recombination: Methods and Protocols is a valuable resource that uses both classical and more recent approaches to answer questions on the HR mechanism. It is a useful tool for scientists working on the field of genome integrity, as well as those working in cancer biology and DNA repair. .

    Online SpringerLink

  2. Hocąk teaching materials. Volume 1, Elements of grammar/learner's dictionary

    Albany : State University of New York Press, ©2010.

    The most comprehensive dictionary of the Hocak language (formerly known as Winncbago) to date, this bidirectional Hocak-English/English-Hocak dictionary contains approximately 4,000 entries. Hocak is a highly endangered North American Indian language spoken by less than two hundred people in different parts of Wisconsin and Nebraska. This dictionary and volume 2 of the Hocak Teaching Materials are the outcome of a large project on the documentation of the Hocak language, which was carried out in close cooperation with the Hocak Language Division, a tribal institution for the stabilization and revitalization of the Hocak language in Mauston, Wisconsin.The volume contains a lengthy introduction to the basics of the phonology, orthography, and morphology of the Hocak language, written in a learner-friendly, easy-to-access style, explaining linguistic terms so that it can be used by nonlinguists. The individual lexical entries of the words are organized according to the standards of modern lexicography, containing all necessary phonetic, grammatical, and semantic information for the use of the Hocak words. In addition, every word is provided with about three Hocak example sentences in order to demonstrate the typical use of the words in different contexts. Also of interest are a frequency list of all words in the dictionary counted on the basis of a large corpus of Hocak texts, and a thesaurus of all Hocak words in the dictionary.A valuable source of information on the Hocak language and culture, this work will appeal to linguists in general, and specialists in Native American languages, as well as anthropologists and all learners of the Hocak language. --Book Jacket.

    Online EBSCO Academic Comprehensive Collection

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